Roles of protein phosphatase 2A in IL-6 signal transduction in Hep3B cells

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dc.contributor.authorIn Pyo Choi-
dc.contributor.authorMin Ju Lee-
dc.contributor.authorEun Joo Kim-
dc.contributor.authorHyung Sik Kang-
dc.contributor.authorKwang Ho Pyun-
dc.date.accessioned2017-04-19T08:55:24Z-
dc.date.available2017-04-19T08:55:24Z-
dc.date.issued1998-
dc.identifier.issn0165-2478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4425-
dc.description.abstractIL-6 is a pleiotropic cytokine that modulates the diverse functions of hepatocytes such as acute phase responses and inflammation. When human hepatoma cells, Hep3B cells, were treated with IL-6, p140 was phosphorylated rapidly and reached its maximal rate at I min after treatment. Okadaic acid, an inhibitor of protein phosphatase 1 and 2A, affected IL-6-induced p140 phosphorylation. Interferon regulatory factor-1 (IRF-1) is a transcription factor on the enhancer of type I intefferons, and its gene expression is induced by IL-6. When IRF-1 promoter-luciferase construct was transfected into Hep3B cells, okadaic acid increased IL-6- induced IRF-1 promoter activity. In addition, co-transfection of protein phosphatase 2A (PP2A) antisense constructs further increased IL-6-induced IRF-1 promoter activity, suggesting that PP2A is involved in IL-6 signaling. In addition, IL-6 directly induced the PP2A phosphorylation. PP2A phosphorylation was maximal at 1 min after IL-6 stimulation, but it was not induced by other inflammatory cytokines such as TNF-α or TGF-β. Furthermore, IL-6 activated PP2A activity simultaneously. Taken together, these data indicate that IL-6 modulates the functions of PP2A which is involved in downstream events of IL-6 signaling in Hep3B.-
dc.publisherElsevier-
dc.titleRoles of protein phosphatase 2A in IL-6 signal transduction in Hep3B cells-
dc.title.alternativeRoles of protein phosphatase 2A in IL-6 signal transduction in Hep3B cells-
dc.typeArticle-
dc.citation.titleImmunology Letters-
dc.citation.number0-
dc.citation.endPage107-
dc.citation.startPage103-
dc.citation.volume61-
dc.contributor.affiliatedAuthorIn Pyo Choi-
dc.contributor.affiliatedAuthorMin Ju Lee-
dc.contributor.affiliatedAuthorHyung Sik Kang-
dc.contributor.affiliatedAuthorKwang Ho Pyun-
dc.contributor.alternativeName최인표-
dc.contributor.alternativeName이민주-
dc.contributor.alternativeName김은주-
dc.contributor.alternativeName강형식-
dc.contributor.alternativeName변광호-
dc.identifier.bibliographicCitationImmunology Letters, vol. 61, pp. 103-107-
dc.identifier.doi10.1016/S0165-2478(98)00005-4-
dc.subject.keywordIL-6-
dc.subject.keywordProtein phosphatase 2A-
dc.subject.keywordIRF-1-
dc.subject.keywordHep3B-
dc.subject.localIL6-
dc.subject.localinterukin -6-
dc.subject.localInterleukin-6 (IL-6)-
dc.subject.localIL-6-
dc.subject.localIl-6-
dc.subject.localinterleukin-6-
dc.subject.localinterleukin-6 (IL-6)-
dc.subject.localInterleukin-6-
dc.subject.localProtein phosphatase 2A (PP2A)-
dc.subject.localProtein phosphatase 2A-
dc.subject.localIRF-1-
dc.subject.localHep3B-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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