Levan and fructosyl derivatives formation by a recombinant levansucrase from Rahnella aquatilis

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Title
Levan and fructosyl derivatives formation by a recombinant levansucrase from Rahnella aquatilis
Author(s)
Min-Gon Kim; Jeong Woo Seo; Ki Bang Song; Chul Ho Kim; Bong Hyun Chung; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 20, no. 4, pp. 333-335
Publication Year
1998
Abstract
Levan, fructo-oligosaccharides and fructosyl derivatives were formed from sucrose using recombinant levansucrase from Rahnella aquatilis. Levan formation was optimal at 30°C resulting 57% of the theoretical yield. The more suitable substrate concentration for levan formation was 200 g sucrose/L. Oligosaccharides was accumulated selectively at high substrate concentration. The increase of levan and oligosaccharides formation was not achieved by adding water-miscible organic solvents. Alkyl fructosides were synthesized from various alcohols as fructosyl accepters by R. aquatilis levansucrase.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005310926399
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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