Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation

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Title
Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation
Author(s)
I G Kim; S K Nam; Jung Hoon Sohn; Sang Ki Rhee; G H An; S H Lee; Eui Sung Choi
Bibliographic Citation
Applied and Environmental Microbiology, vol. 64, no. 5, pp. 1947-1949
Publication Year
1998
Abstract
The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/μg of DNA with an integrated copy number of about seven per haploid genome.
ISSN
0099-2240
Publisher
Amer Soc Microb
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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