Open Access@KRIBBSynthetic Biology and Bioengineering Research InstituteSynthetic Biology Research Center1. Journal Articles
Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation
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- Title
- Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation
- Author(s)
- I G Kim; S K Nam; Jung Hoon Sohn; Sang Ki Rhee; G H An; S H Lee; Eui Sung Choi
- Bibliographic Citation
- Applied and Environmental Microbiology, vol. 64, no. 5, pp. 1947-1949
- Publication Year
- 1998
- Abstract
- The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/μg of DNA with an integrated copy number of about seven per haploid genome.
- ISSN
- 0099-2240
- Publisher
- Amer Soc Microb
- Type
- Article
- Appears in Collections:
- Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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