Synthetic peptides of human papillomavirus type 18 E6 harboring HLA-A2.1 motif can induce peptide-specific cytotoxic T-cells from peripheral blood mononuclear cells of healthy donors

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Title
Synthetic peptides of human papillomavirus type 18 E6 harboring HLA-A2.1 motif can induce peptide-specific cytotoxic T-cells from peripheral blood mononuclear cells of healthy donors
Author(s)
Hee Sik Yoon; Mi Kyung Chung; Sung Shik Min; Hee Gu Lee; Wang Don Yoo; Keun Taek Chung; Noh Pal Jung; Sue Nie Park
Bibliographic Citation
Virus Research, vol. 54, pp. 23-29
Publication Year
1998
Abstract
To identify cytotoxic T-cell (CTL) epitopes against human papillomavirus type 18 (HPV 18) E6 protein that might be useful for developing peptide-based vaccine against HPV 18 infection, 18 peptides which possibly contain CTL epitopes were selected on the basis of previously described human leukocyte antigen (HLA)-A2.1-binding motif and chemically synthesized. In the binding assay of the synthetic peptides, 8 out of 18 synthetic peptides enhanced the expression of HLA-A2.1 molecules on T2 cell surface, which implies that these peptides were able to bind the HLA molecules. Those peptides having good binding affinity to HLA-A2.1 were tested for their ability to activate CTLs which were isolated from peripheral blood mononuclear cells (PBMCs) of healthy blood donors and to kill the target T2 cells pulsed with the same peptide. Five out of eight tested peptides activated CTLs and killed the target cells.
Keyword
Human papillomavirus type 18E6 oncoproteinHuman leukocyte antigen-A2.1Epitope peptideCytotoxic T-lymphocytes
ISSN
0168-1702
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0168-1702(98)00008-2
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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