Isolation and structural determination of anti-Helicobacter pylori compound from fungus 60686 = 곰팡이에서 분리한 Helicobacter pylori 항균물질의 분리 및 구조규명

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Title
Isolation and structural determination of anti-Helicobacter pylori compound from fungus 60686 = 곰팡이에서 분리한 Helicobacter pylori 항균물질의 분리 및 구조규명
Author(s)
June Namgoong; Seung Woo Yeon; Nam Soo Paek; Tae Han Kim; Young Ho Kim; Chang Jin Kim; Kee Won Kim
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 26, no. 2, pp. 137-142
Publication Year
1998
Abstract
Helicobacter pylori is a Gram-negative bacterium which causes chronic gastritis and is associated with gastric ulcer, duodenal ulcer and gastric carcinoma. In the process of screening of antibacterial activities against H. pylori from soil microorganisms, fungus No. 60686 was isolated. After fermentation of No.60686, the antibacterial compound was isolated, purified and followed by extraction of mycelium with organic solvents, acetone and ethyl acetate, through silica gel chromatography, LH-20 gel chromatography and HPLC. As a result of the structural analyses of the compound by IR, 1H- and 13C-NMR, FAB/Mass spectrophotomer, the compound having the antimicrobial activity was identified as chaetoglobosin A (C32H36N2O5), a cytochalasan derivative. The antimicrobial activity of chaetoglobosin A was tested against Gram-positive and negative bacteria by paper disk method. Among the test strains of 9 Gram-positive bacteria and 18 Gram-negative bacteria containing 4 H. pylori strains, the growth of 4 H. pylori strains and 3 S. aureus strains (SG 511, 285 and 503) was only inhibited by chaetoglobosin A. Also it was shown that its growth inhibition against H. pylori strains was stronger than that against S. aureus strains at the treatment of the same concentration. Therefore it was concluded that chaetoglobosin A has a specific growth inhibition against H. pylori of the tested bacteria.
Keyword
Helicobacter pyloriChaetoglobosin Aantimicrobial activity
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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