Rapid and simple purification of biologically active human hepatitis B virus transactivator-X proteins

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dc.contributor.authorHa Ryoung Poo-
dc.contributor.authorSun Ok Kim-
dc.contributor.authorMi Jin Sohn-
dc.contributor.authorSook Lee-
dc.contributor.authorYoung Ik Lee-
dc.date.accessioned2017-04-19T08:55:31Z-
dc.date.available2017-04-19T08:55:31Z-
dc.date.issued1998-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4478-
dc.description.abstractThe human hepatitis B virus-X (HBV-X) was cloned into an expression vector, pET3d, containing a T7 promoter and direct expression was induced in Escherichia coli. The expressed HBV-X protein was purified to homogeneity by centrifugation, ion-exchange chromatography and gel filtration. After gel filtration, renaturation of HBV-X were performed using dialysis against serially diluted urea buffer. The biological activity of refolded HBV-X protein was confirmed by enhancement of protein/DNA complexes using gel-shift analysis.-
dc.publisherMicrobiological Society Korea-
dc.titleRapid and simple purification of biologically active human hepatitis B virus transactivator-X proteins-
dc.title.alternativeRapid and simple purification of biologically active human hepatitis B virus transactivator-X proteins-
dc.typeArticle-
dc.citation.titleJournal of Microbiology-
dc.citation.number1-
dc.citation.endPage58-
dc.citation.startPage55-
dc.citation.volume36-
dc.contributor.affiliatedAuthorHa Ryoung Poo-
dc.contributor.affiliatedAuthorSun Ok Kim-
dc.contributor.affiliatedAuthorSook Lee-
dc.contributor.affiliatedAuthorYoung Ik Lee-
dc.contributor.alternativeName부하령-
dc.contributor.alternativeName김선옥-
dc.contributor.alternativeName손미진-
dc.contributor.alternativeName이숙-
dc.contributor.alternativeName이영익-
dc.identifier.bibliographicCitationJournal of Microbiology, vol. 36, no. 1, pp. 55-58-
dc.subject.keywordHepatitis B virus-X protein-
dc.subject.keywordexpression-
dc.subject.keywordpurification-
dc.subject.localhepatitis B virus X protein-
dc.subject.localHepatitis B virus-X protein-
dc.subject.localHepatitis B virus X protein-
dc.subject.localhepatitis B virus-X protein-
dc.subject.localHepatitis B virus X-protein-
dc.subject.localexpression-
dc.subject.localExpression-
dc.subject.localPurification-
dc.subject.localpurification-
dc.subject.localPurifcation-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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