Jacalin interacts with Asn-linked glycopeptides containing multiantennary oligosaccharide structure with terminalα-linked galactose

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Title
Jacalin interacts with Asn-linked glycopeptides containing multiantennary oligosaccharide structure with terminalα-linked galactose
Author(s)
Su Il Do; Ki-Young Lee
Bibliographic Citation
FEBS Letters, vol. 421, pp. 169-173
Publication Year
1998
Abstract
The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamineradiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of α-galactosidase whereas O-linked glycopeptides were still bound lectin after this treatment. The removal of O-linked oligosaccharides by mild alkaline/borohydride treatment completely eliminated the lectin binding of α-galactosidase treated glycopeptides. These results demonstrate that jacalin interacts with cellular glycopeptides containing N-linked oligosaccharides with terminal α- galactose residues as well as glycopeptides containing O-linked oligosaccharides.
Keyword
Jacalin lectinRAF9 cell-derived N-linked glycopeptideTerminal α- galactose residueα-Galactosidase
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(97)01539-1
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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