Identification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe

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dc.contributor.authorKwang Lae Hoe-
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorMi Sun Won-
dc.contributor.authorO J Yoo-
dc.contributor.authorHyang Sook Yoo-
dc.description.abstractBy genomic Southern hybridization using degenerate oligo probes corresponding to the conserved zinc finger of the GATA family, four and three new GATA-factor genes were detected in Saccharomyces cereviaiae and Schizosaccharomyces and pombe respectively. Through degenerate PCR and Southern hybridization using an internal probe between the PCR primers, a new GATA-factor gene of S. pombe, gaf1, was identified. The DNA sequence analysis revealed that the partial gafl gene contains the strictly conserved zinc finger which is the hallmark of the GATA family. As judged form the codon usage of leucine in the zinc finger probe, the zinc fingers of S. pombe are more similar to those of higher fungi than those of S. cerevisiae, and this means that S. pombe is phylogenically close to higher organisms than S. cerevisiae.-
dc.titleIdentification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe-
dc.title.alternativeIdentification of new GATA factor family genes in Saccharomyces cerevisiae & Schizosaccharomyces pombe-
dc.citation.titleBiochemistry and Molecular Biology International-
dc.contributor.affiliatedAuthorKwang Lae Hoe-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.affiliatedAuthorHyang Sook Yoo-
dc.identifier.bibliographicCitationBiochemistry and Molecular Biology International, vol. 44, no. 5, pp. 897-906-
dc.subject.keywordGATA factor-
dc.subject.keywordzinc finger-
dc.subject.keywordS. pombe-
dc.subject.keyworddegenerate PCR-
dc.subject.localGATA factor-
dc.subject.localZinc finger-
dc.subject.localzinc finger-
dc.subject.localS. pombe-
dc.subject.localDegenerate PCR-
dc.subject.localdegenerate PCR-
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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