Efficient in vitro model for immunotoxicologic assessment of mammary silicone implants

Cited 5 time in scopus
Metadata Downloads
Title
Efficient in vitro model for immunotoxicologic assessment of mammary silicone implants
Author(s)
J W Rhie; Sang Bae Han; J H Byeon; S T Ahn; Hwan Mook Kim
Bibliographic Citation
Plastic and Reconstructive Surgery, vol. 102, no. 1, pp. 73-77
Publication Year
1998
Abstract
In clinical and experimental studies, silicone gel has been assumed to cause immune alterations that may be related to macrophage activation of silicone implants. However, it has not been proven that the immunotoxicities are caused by the direct contact of macrophages and silicone gel because there has not been an adequate experimental model. In the present study, silicone gel was loaded directly onto Petri dishes and was distributed uniformly to the bottom by centrifugation. Peritoneal macrophages and splenic lymphocytes were cultured either on the silicone-coated plates or on the conventional, normal plates, and their functions were compared with each other. The experiments were repeated three times. The cytotoxic activities of peritoneal macrophages on cancer cells were markedly augmented by cultivation on silicone gel, and the primary T-dependent immunoglobulin M response in which macrophages participated as antigen presenting cells was also enhanced by incubation on silicone gel. However, macrophage-unrelated functions mediated by B and T lymphocytes were not affected by the silicone gel treatment. It was proven that the direct contact of macrophages with silicone gel was a primary cause of acute immune activation that was related to foreign body reaction. In addition, the present in vitro model exhibited similar silicone-induced immunotoxicities in previous animal and clinical studies.
ISSN
0032-1052
Publisher
Kluwer
DOI
http://dx.doi.org/10.1097/00006534-199807000-00011
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.