Expression of thermostable alkaline protease gene from Thermoactinomyces sp. E79 in E. coli and heat activation of the gene product

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Title
Expression of thermostable alkaline protease gene from Thermoactinomyces sp. E79 in E. coli and heat activation of the gene product
Author(s)
Jung Kee Lee; Young Ok Kim; K Sunitha; Tae Kwang Oh
Bibliographic Citation
Biotechnology Letters, vol. 20, no. 9, pp. 837-840
Publication Year
1998
Abstract
The expression of Thermoactinomyces sp. E79 protease gene cloned into E. coli was highly host-dependent and the levels of protease expression was most stable in E. coli RR1 and E. coli HB101. Heating the intracellular extract at 70°C for 15 min converted the inactive recombinant E79 protease to its active mature form and also resulted in purification of the enzyme in a single step. Addition of 10 mM CaCl2 to the E79 protease decreased its autolysis and increased its thermal stability.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005355324065
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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