Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene

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dc.contributor.authorKwang Hyun Ah-
dc.contributor.authorJeong Yoon Kim-
dc.contributor.authorSu Min Ko-
dc.contributor.authorCheon Seok Park-
dc.contributor.authorDewey D Y Ryu-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:55:39Z-
dc.date.available2017-04-19T08:55:39Z-
dc.date.issued1998-
dc.identifier.issn0749-503X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4533-
dc.description.abstractA gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPases. The cloned gene product exhibits 60.3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevisiae PMR1 gene product, a P-type Ca2+-ATPase. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession Number U92083.-
dc.publisherWiley-
dc.titleCloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene-
dc.title.alternativeCloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene-
dc.typeArticle-
dc.citation.titleYeast-
dc.citation.number0-
dc.citation.endPage1240-
dc.citation.startPage1233-
dc.citation.volume14-
dc.contributor.affiliatedAuthorKwang Hyun Ah-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName김정윤-
dc.contributor.alternativeName고수민-
dc.contributor.alternativeName박천석-
dc.contributor.alternativeName유도영-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationYeast, vol. 14, pp. 1233-1240-
dc.identifier.doi10.1002/(SICI)1097-0061(19980930)14:13<1233::AID-YEA322>3.0.CO;2-Y-
dc.subject.keywordCa2+-ATPase-
dc.subject.keywordHansenula polymorpha-
dc.subject.keywordPMR1-
dc.subject.keywordYeast-
dc.subject.localCa2+-ATPase-
dc.subject.localHansenulapolymorpha-
dc.subject.localhansenula polymorpha-
dc.subject.localHansenula polymorpha-
dc.subject.localHansenula polymorpha (Pichia angusta)-
dc.subject.localPMR1-
dc.subject.localYeast-
dc.subject.localyeasts-
dc.subject.localyeast-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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