An industrial medium for improved production of carotenoids from a mutant strain of Phaffia rhodozyma

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Title
An industrial medium for improved production of carotenoids from a mutant strain of Phaffia rhodozyma
Author(s)
S Siva Kesava; Gil Hwan An; Chul Ho Kim; Sang Ki Rhee; Eui Sung Choi
Bibliographic Citation
Bioprocess Engineering, vol. 19, pp. 165-170
Publication Year
1998
Abstract
A medium based on less expensive nutrient sources, such as corn starch hydrolyzate (hydrol), corn steep liquor (CSL), urea and potassium phosphate was used for the growth of the yeast Phaffia rhodozyma 2A2N strain. A central composite experimental design has been employed to derive a statistical model on the effect of hydrol and CSL on carotenoid production. An initial concentration of sugars as glucose equivalent 73 g/l in hydrol and 43 g/l CSL were found optimal for the maximization of final carotenoid production in shake flask cultures. The carotenoid production was increased by adding urea and phosphate sources. Laboratory scale fermentation was performed with the optimized medium and total carotenoid production of 52.4 mg/l was obtained using constant fed-batch culture.
Keyword
CarotenoidPotassium PhosphateCorn StarchTotal CarotenoidCorn Steep Liquor
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/PL00009009
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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