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- An industrial medium for improved production of carotenoids from a mutant strain of Phaffia rhodozyma
- S Siva Kesava; Gil Hwan An; Chul Ho Kim; Sang Ki Rhee; Eui Sung Choi
- Bibliographic Citation
- Bioprocess Engineering, vol. 19, pp. 165-170
- Publication Year
- A medium based on less expensive nutrient sources, such as corn starch hydrolyzate (hydrol), corn steep liquor (CSL), urea and potassium phosphate was used for the growth of the yeast Phaffia rhodozyma 2A2N strain. A central composite experimental design has been employed to derive a statistical model on the effect of hydrol and CSL on carotenoid production. An initial concentration of sugars as glucose equivalent 73 g/l in hydrol and 43 g/l CSL were found optimal for the maximization of final carotenoid production in shake flask cultures. The carotenoid production was increased by adding urea and phosphate sources. Laboratory scale fermentation was performed with the optimized medium and total carotenoid production of 52.4 mg/l was obtained using constant fed-batch culture.
- Carotenoid; Potassium Phosphate; Corn Starch; Total Carotenoid; Corn Steep Liquor
- Appears in Collections:
- Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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