Fermentation strategy to enhance plasmid stability during the cultivation of Escherichia coli for the production of recombinant levansucrase

Cited 7 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorChul Ho Kim-
dc.contributor.authorJang Young Lee-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorKi Bang Song-
dc.contributor.authorJeong Woo Seo-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorSoon Jae Chang-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:55:39Z-
dc.date.available2017-04-19T08:55:39Z-
dc.date.issued1998-
dc.identifier.issn0385-6380-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4536-
dc.description.abstractTo produce levansucrase, a fructosyltransferase enzyme, in a recombinant Escherichia coli harboring the levU gene of Zymomonas mobilis, fermentation strategies were examined in terms of induction methods and plasmid stability. Although the recombinant levansucrase was induced rapidly by IPTG, high instability of the plasmid and formation of inclusion bodies were observed. Segregational instability was aggravated by the excretion of β-lactamase into the culture broth during subculturing, which caused an overgrowth of plasmid-free cells. A combination of methicillin (2, 6-dimethoxyphenyl- penicillin) and ampicillin to provide selective pressure was effective in preventing the growth of plasmid-free cells. The population of plasmid- harboring cells was maintained above 95% of the total cells for more than 100 generations under this condition. In order to replace IPTG, which is toxic and too expensive for use in a large scale, D-lactose was tested and found to be favorable as an alternative inducer.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titleFermentation strategy to enhance plasmid stability during the cultivation of Escherichia coli for the production of recombinant levansucrase-
dc.title.alternativeFermentation strategy to enhance plasmid stability during the cultivation of Escherichia coli for the production of recombinant levansucrase-
dc.typeArticle-
dc.citation.titleJournal of Fermentation Bioengineering-
dc.citation.number4-
dc.citation.endPage394-
dc.citation.startPage391-
dc.citation.volume86-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.affiliatedAuthorKi Bang Song-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName이장영-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName송기방-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName장순재-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationJournal of Fermentation Bioengineering, vol. 86, no. 4, pp. 391-394-
dc.identifier.doi10.1016/S0922-338X(99)89010-2-
dc.subject.keywordLevansucrase-
dc.subject.keywordZymomonas mobilis-
dc.subject.keywordplasmid stability-
dc.subject.keywordinduction methods-
dc.subject.localLevansucrase-
dc.subject.locallevansucrase-
dc.subject.localzymomonas mobilis-
dc.subject.localZYMOMONAS MOBILIS-
dc.subject.localZymomonas mobilis-
dc.subject.localplasmid stability-
dc.subject.localinduction methods-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.