DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chul Ho Kim | - |
dc.contributor.author | Jang Young Lee | - |
dc.contributor.author | Min-Gon Kim | - |
dc.contributor.author | Ki Bang Song | - |
dc.contributor.author | Jeong Woo Seo | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | Soon Jae Chang | - |
dc.contributor.author | Sang Ki Rhee | - |
dc.date.accessioned | 2017-04-19T08:55:39Z | - |
dc.date.available | 2017-04-19T08:55:39Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0385-6380 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4536 | - |
dc.description.abstract | To produce levansucrase, a fructosyltransferase enzyme, in a recombinant Escherichia coli harboring the levU gene of Zymomonas mobilis, fermentation strategies were examined in terms of induction methods and plasmid stability. Although the recombinant levansucrase was induced rapidly by IPTG, high instability of the plasmid and formation of inclusion bodies were observed. Segregational instability was aggravated by the excretion of β-lactamase into the culture broth during subculturing, which caused an overgrowth of plasmid-free cells. A combination of methicillin (2, 6-dimethoxyphenyl- penicillin) and ampicillin to provide selective pressure was effective in preventing the growth of plasmid-free cells. The population of plasmid- harboring cells was maintained above 95% of the total cells for more than 100 generations under this condition. In order to replace IPTG, which is toxic and too expensive for use in a large scale, D-lactose was tested and found to be favorable as an alternative inducer. | - |
dc.publisher | Soc Bioscience Bioengineering Japan | - |
dc.title | Fermentation strategy to enhance plasmid stability during the cultivation of Escherichia coli for the production of recombinant levansucrase | - |
dc.title.alternative | Fermentation strategy to enhance plasmid stability during the cultivation of Escherichia coli for the production of recombinant levansucrase | - |
dc.type | Article | - |
dc.citation.title | Journal of Fermentation Bioengineering | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 394 | - |
dc.citation.startPage | 391 | - |
dc.citation.volume | 86 | - |
dc.contributor.affiliatedAuthor | Chul Ho Kim | - |
dc.contributor.affiliatedAuthor | Min-Gon Kim | - |
dc.contributor.affiliatedAuthor | Ki Bang Song | - |
dc.contributor.affiliatedAuthor | Jeong Woo Seo | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.affiliatedAuthor | Sang Ki Rhee | - |
dc.contributor.alternativeName | 김철호 | - |
dc.contributor.alternativeName | 이장영 | - |
dc.contributor.alternativeName | 김민곤 | - |
dc.contributor.alternativeName | 송기방 | - |
dc.contributor.alternativeName | 서정우 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 장순재 | - |
dc.contributor.alternativeName | 이상기 | - |
dc.identifier.bibliographicCitation | Journal of Fermentation Bioengineering, vol. 86, no. 4, pp. 391-394 | - |
dc.identifier.doi | 10.1016/S0922-338X(99)89010-2 | - |
dc.subject.keyword | Levansucrase | - |
dc.subject.keyword | Zymomonas mobilis | - |
dc.subject.keyword | plasmid stability | - |
dc.subject.keyword | induction methods | - |
dc.subject.local | Levansucrase | - |
dc.subject.local | levansucrase | - |
dc.subject.local | zymomonas mobilis | - |
dc.subject.local | ZYMOMONAS MOBILIS | - |
dc.subject.local | Zymomonas mobilis | - |
dc.subject.local | plasmid stability | - |
dc.subject.local | induction methods | - |
dc.description.journalClass | Y | - |
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