Effect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae

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dc.contributor.authorK Janannadha Rao-
dc.contributor.authorChul Ho Kim-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:55:40Z-
dc.date.available2017-04-19T08:55:40Z-
dc.date.issued1998-
dc.identifier.issn0178-515X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4537-
dc.description.abstractDifferent feeding strategies of galactose were employed to improve the production of anticoagulant, hirudin, by fed-batch mode of cultivation from recombinant Saccharomyces cerevisiae. The structural gene coding for hirudin was harboured with GAL10 promoter for controlled expression of hirudin and the MFα 1 signal sequence for secretion into the growth medium. A stepwise feeding of galactose was found as more suitable feeding strategy of galactose which resulted in the final hirudin volumetric productivity of 6,840 μg/l · h, than intermittent, continuous and ethanol controlled feeding of galactose. The final volumetric productivity of hirudin obtained by step-wise feeding of galactose was 3.88 fold higher compared with simple batch fermentation.-
dc.publisherSpringer-
dc.titleEffect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae-
dc.title.alternativeEffect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinant Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleBioprocess Engineering-
dc.citation.number5-
dc.citation.endPage388-
dc.citation.startPage385-
dc.citation.volume19-
dc.contributor.affiliatedAuthorChul Ho Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeNameRao-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationBioprocess Engineering, vol. 19, no. 5, pp. 385-388-
dc.identifier.doi10.1007/s004490050536-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
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