Identification of a new 5'-noncoding exon region and promoter activity in human N-acetylglucosaminyltransferase III gene

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dc.contributor.authorBong Seok Kang-
dc.contributor.authorYeon Jeong Kim-
dc.contributor.authorJae Kyoung Shim-
dc.contributor.authorEun Young Song-
dc.contributor.authorYoung Guk Park-
dc.contributor.authorYoung Choon Lee-
dc.contributor.authorKyung Soo Nam-
dc.contributor.authorJune Ki Kim-
dc.contributor.authorTae Kyun Lee-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorCheorl Ho Kim-
dc.date.accessioned2017-04-19T08:55:40Z-
dc.date.available2017-04-19T08:55:40Z-
dc.date.issued1998-
dc.identifier.issn12258687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4540-
dc.description.abstractIn a previous paper (Kim et al., 1996a), the immediate 5′-flanking region and coding region of the human UDP-N-acetylglucosamine:-D-mannoside-1,4-N-acetylglucosaminyltransferase III (N-acetylglucosaminyltransferase-III; GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5′-noncoding region of the GnT-III mRNA by single-strand ligation to single-stranded cDNA-PCR (5′-RACE PCR) using poly(A)+ RNA isolated from human fetal liver cells. A cDNA clone was obtained with 5′ sequences (96 bp) that diverged seven nucleotides upstream from the ATG (+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5′ to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5′-noncoding region and that the cDNA was an incompletely reverse-transcribed cDNA product derived from an mRNA containing a new noncoding exon. When mRNA expression of GnT-III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT-III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5′-flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2, by luciferase assays. The 5′-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.-
dc.publisherSouth Korea-
dc.titleIdentification of a new 5'-noncoding exon region and promoter activity in human N-acetylglucosaminyltransferase III gene-
dc.title.alternativeIdentification of a new 5'-noncoding exon region and promoter activity in human N-acetylglucosaminyltransferase III gene-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number6-
dc.citation.endPage584-
dc.citation.startPage578-
dc.citation.volume31-
dc.contributor.alternativeName강봉석-
dc.contributor.alternativeName김연정-
dc.contributor.alternativeName심재경-
dc.contributor.alternativeName송은영-
dc.contributor.alternativeName박영국-
dc.contributor.alternativeName이영춘-
dc.contributor.alternativeName남경수-
dc.contributor.alternativeName김준기-
dc.contributor.alternativeName이태균-
dc.contributor.alternativeName정태화-
dc.contributor.alternativeName김철호-
dc.identifier.bibliographicCitationBMB Reports, vol. 31, no. 6, pp. 578-584-
dc.subject.keywordD-N-acetylglucosamine:-D-mannoside-1,4-N-acetylglucosaminyltransferase III-
dc.subject.keywordExpression-
dc.subject.keywordPromoter-
dc.subject.localD-N-acetylglucosamine:-D-mannoside-1,4-N-acetylglucosaminyltransferase III-
dc.subject.localExpression-
dc.subject.localPromoter-
dc.description.journalClassY-
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