Molecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator

Cited 12 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKwang Lae Hoe-
dc.contributor.authorMi Sun Won-
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorSeung Kiel Park-
dc.contributor.authorDong Uk Kim-
dc.contributor.authorYoung Joo Jang-
dc.contributor.authorOok Joon Yoo-
dc.contributor.authorHyang Sook Yoo-
dc.date.accessioned2017-04-19T08:55:40Z-
dc.date.available2017-04-19T08:55:40Z-
dc.date.issued1998-
dc.identifier.issn0378-1119-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4541-
dc.description.abstractAs a first step to elucidate the functions of Schizosaccharomyces pombe (S. pombe) GATA factors, we have isolated the gaf1+ gene (GATA-factor like gene) in S. pombe. The predicted amino acid (aa) sequence of Gaf1 reveals a single zinc finger domain typical of fungal GATA factors, and the zinc finger exhibits 60% aa identity to that of human GATA-1. The open reading frame of Gaf1 predicts a protein of M(r) 32 kDa consisting of 290 intronless amino acids. Disruption of this gene has no effect on cell viability and growth rate. The GST-Gaf1 fusion protein binds specifically to GATA motifs of its own promoter as well as DAL7 UAS, a canonical GATA motif of Saccharomyces cerevisiae (S. cerevisiae) The specific DNA-binding activity resides within the N-terminal half of Gaf1 (Gaf1N; aa 1-120) containing the zinc finger, whereas the C-terminal half (Gaf1C; aa 121-290) contains transactivation sequences that induce the expression of the lacZ reporter when fused to the GAL4 DNA binding domain. These results demonstrate that Gaf1 may function as a transcriptional activator consisting of DNA-binding and transactivation domains.-
dc.publisherElsevier-
dc.titleMolecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator-
dc.title.alternativeMolecular cloning of Gaf1, a Schizosaccharomyces pombe GATA factor, which can function as a transcriptional activator-
dc.typeArticle-
dc.citation.titleGene-
dc.citation.number2-
dc.citation.endPage328-
dc.citation.startPage319-
dc.citation.volume215-
dc.contributor.affiliatedAuthorKwang Lae Hoe-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorSeung Kiel Park-
dc.contributor.affiliatedAuthorDong Uk Kim-
dc.contributor.affiliatedAuthorYoung Joo Jang-
dc.contributor.affiliatedAuthorHyang Sook Yoo-
dc.contributor.alternativeName허광래-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName박승길-
dc.contributor.alternativeName김동욱-
dc.contributor.alternativeName장영주-
dc.contributor.alternativeName유욱준-
dc.contributor.alternativeName유향숙-
dc.identifier.bibliographicCitationGene, vol. 215, no. 2, pp. 319-328-
dc.identifier.doi10.1016/S0378-1119(98)00301-1-
dc.subject.keywordZinc finger-
dc.subject.keywordGATA-binding-
dc.subject.keywordTranscription factor-
dc.subject.keywordDegenerate PCR-
dc.subject.keywordS. pombe-
dc.subject.localZinc finger-
dc.subject.localzinc finger-
dc.subject.localGATA-binding-
dc.subject.localtranscription factor-
dc.subject.localTranscription factors-
dc.subject.localtranscription factors-
dc.subject.localTranscription factor-
dc.subject.localDegenerate PCR-
dc.subject.localdegenerate PCR-
dc.subject.localS. pombe-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.