Production and rheological properties of bioflocculant produced by Bacillus sp. DP-152

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Production and rheological properties of bioflocculant produced by Bacillus sp. DP-152
Hyun Hyo Suh; Seong Hoon Moon; Hee Sik Kim; Hyoung Kab Kim; Gee Ill Jun; Hyun Geoun Park; Dae Ook Kang; Hee Moak Oh; Byung Dae Yoon
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 8, no. 6, pp. 618-624
Publication Year
The culture conditions for Bacillus sp. DP-152 in the flask were investigated for the production of polysaccharide flocculant, DP-152. The optimum pH and temperature for the flocculant production were 8.0 and 30°(Degree Sign)C, respectively. The favorable substrates for flocculant production were soluble starch and ammonium nitrate. The medium composition was optimized as follows: 30 g soluble starch, 0.75 g NH4NO3, 2.0 g K2HPO4, 0.1 g KH2PO4, 0.2 g MgSO4·7H2O, and 0.2 g MnSO4·4~5H2O in 11 of distilled water. Under this optimized condition, flocculating activity has been improved 4-fold compared with that of the basal medium. In the culture flask, the highest flocculating activity was obtained after 70 h of cultivation and the amount of bioflocculant DP-152 yielded was 12.4 g/1. The solution of bioflocculant DP-152 showed non-Newtonian characteristics. Bioflocculant DP-152 exhibited apparently higher viscosity at all concentrations compared to that of zooglan (from Zoogloea ramigera), and it was stable over a wide range of temperatures and pHs.
Bacillus speciesbioflocculant productionrheology
Korea Soc-Assoc-Inst
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Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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