Roles of acetyl-CoA carboxylase β in muscle cell differentiation and in mitochondrial fatty acid oxidation

Abstract

Acetyl-CoA carboxylase β is expressed primarily in heart and skeletal muscle, where it may play a role in the control of mitochondrial fatty acid uptake and oxidation by controlling carnitine-palmitoyl-CoA transferase 1. To investigate the relationship between ACC-β expression and myoblast differentiation, we specifically blocked ACC-β production by expressing an antisense gene to the specific N-terminal region of ACC-β in H9c2 cells. The expression of the antisense ACC-β mRNA retarded cell fusion, myosin formation, and creatine kinase induction; all of these parameters are hallmarks of muscle cell differentiation. On the other hand, the rate of fatty acid oxidation was not affected by the presence or absence of ACC-β in the cells.