Differentially expressed aortic genes in cholesterol-fed rabbits

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dc.contributor.authorTae Gyun Kim-
dc.contributor.authorMoon Kyoo Jang-
dc.contributor.authorNam Ho Jeoung-
dc.contributor.authorMyung Sook Choi-
dc.contributor.authorSong Hae Bok-
dc.contributor.authorJu Won Kwak-
dc.contributor.authorYong Bok Park-
dc.date.accessioned2017-04-19T08:55:46Z-
dc.date.available2017-04-19T08:55:46Z-
dc.date.issued1998-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4585-
dc.description.abstractIn order to identify key genes involved in the development of atherosclerotic lesions, differentially expressed genes in atherosclerotic plaques obtained from diet-induced hypercholesterolemic rabbit aorta were screened using the differential display (DD) RT-PCR technique. Aortic RNAs were isolated from rabbits fed cholesterol-supplemented (2% cholesterol in lab-chow, w/w) chow diet for 12 weeks, followed by the synthesis of cDNAs by reverse-transcription using 2-base anchored oligo(dT) (5′-T11VN) as 3′-primers. Synthesized cDNAs were amplified by PCR using arbitrary 10-mers as 5′-primers and the same 3′-primers used in the reverse-transcription. Amplified cDNAs sized between 0.2 to 0.5 kb obtained from control and cholesterol-fed rabbit aortas were displayed on the 6% DNA-sequencing gel for comparisons. The cDNA bands showing distinctive differences in patterns of display or in density of the band were extracted from the gel. A total of 66 differentially displayed cDNAs was isolated and subjected to the reverse-Northern and Northern blot analyses in order to confirm the differences. Through the extensive confirming processes, three cDNAs were finally selected (designated CRGRA-1 through -3) and their nucleotide sequences were determined. Two of those (CRGRA-1 and -2) were determined to be up-regulated and the other (CRGRA-3) was down-regulated by the cholesterol-feeding. Upon homology search on databases for the identification of the genes, the first cDNA (CRGRA-1) turned out to be a part of a novel gene, the second one (CRGRA-2) was homologous (82%) to the corresponding segment of mitochondrial NADH dehydrogenase subunit 4 gene, and the last one (CRGRA-3) was identified to be homologous (94%) to a segment of human small GTP-binding protein (Rab7) gene.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDifferentially expressed aortic genes in cholesterol-fed rabbits-
dc.title.alternativeDifferentially expressed aortic genes in cholesterol-fed rabbits-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number3-
dc.citation.endPage329-
dc.citation.startPage324-
dc.citation.volume8-
dc.contributor.affiliatedAuthorSong Hae Bok-
dc.contributor.affiliatedAuthorJu Won Kwak-
dc.contributor.alternativeName김태균-
dc.contributor.alternativeName장문규-
dc.contributor.alternativeName정남호-
dc.contributor.alternativeName최명숙-
dc.contributor.alternativeName복성해-
dc.contributor.alternativeName곽주원-
dc.contributor.alternativeName박용복-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 8, no. 3, pp. 324-329-
dc.subject.keywordAtherosclerosis-
dc.subject.keywordAtherosclerotic Lesion-
dc.subject.keywordCholesterol-
dc.subject.keywordDDRT-PCR-
dc.subject.localatherosclerosis-
dc.subject.localAtherosclerosis-
dc.subject.localatheroclerosis-
dc.subject.localAtherosclerotic Lesion-
dc.subject.localcholesterol-
dc.subject.localCholesterol-
dc.subject.localDDRT-PCR-
dc.description.journalClassY-
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