Construction of a chimeric series of Bacillus cyclomaltodextrin glucanotransferases and analysis of the thermal stabilities and pH optima of the enzymes

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dc.contributor.authorTakahiro Kaneko-
dc.contributor.authorKi Bang Song-
dc.contributor.authorTetsuo Hamamoto-
dc.contributor.authorToshiaki Kudo-
dc.contributor.authorKoki Horikoshi-
dc.date.accessioned2017-04-19T08:55:47Z-
dc.date.available2017-04-19T08:55:47Z-
dc.date.issued1989-
dc.identifier.issn0022-1287-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4590-
dc.description.abstractThe cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from the alkalophilic Bacillus sp. strain no. 17-1 was cloned in Escherichia coli. The cloned CGTase gene consisted of single open reading frame which would encode a polypeptide of 713 amino acids, and the first 27 amino acid residues comprised a signal peptide. The nucleotide sequence and the amino acid sequence of this CGTase (CGTase) 17-1) gene had strong homology with those of the CGTase (CGTase 38-2) gene previously cloned in our laboratory from the alkalophilic Bacillus sp. strain no. 38-2, although the enzymic properties of the CGTase 17-1 were distinct from those of the CGTase 38-2. To analyse those enzymic properties further, we constructed 12 chimeric CGTases using three restriction nuclease sites and compared the enzymic properties of the chimeric CGTases. The N-terminal part of the enzyme was important for heat stability, and the pH-activity profile was influenced by both the N- and the C-terminal parts. A third segment was less important for enzymic properties.-
dc.publisherMicrobiology Soc-
dc.titleConstruction of a chimeric series of Bacillus cyclomaltodextrin glucanotransferases and analysis of the thermal stabilities and pH optima of the enzymes-
dc.title.alternativeConstruction of a chimeric series of Bacillus cyclomaltodextrin glucanotransferases and analysis of the thermal stabilities and pH optima of the enzymes-
dc.typeArticle-
dc.citation.titleJournal of General Microbiology-
dc.citation.number0-
dc.citation.endPage3457-
dc.citation.startPage3447-
dc.citation.volume135-
dc.contributor.affiliatedAuthorKi Bang Song-
dc.contributor.alternativeNameKaneko-
dc.contributor.alternativeName송기방-
dc.contributor.alternativeNameHamamoto-
dc.contributor.alternativeNameKudo-
dc.contributor.alternativeNameHorikoshi-
dc.identifier.bibliographicCitationJournal of General Microbiology, vol. 135, pp. 3447-3457-
dc.identifier.doi10.1099/00221287-135-12-3447-
dc.description.journalClassY-
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