Structure of β-galactosidase gene from Streptococcus thermophilus SKD 1006 = Streptococcus thermophilus SKD 1006의 β-Galactosidase 유전자 구조

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Title
Structure of β-galactosidase gene from Streptococcus thermophilus SKD 1006 = Streptococcus thermophilus SKD 1006의 β-Galactosidase 유전자 구조
Author(s)
Hae Ki Min; Kook Hee Kang; Moon Hee Sung
Bibliographic Citation
성균관대학교논문집-과학기술편, vol. 43, no. 2, pp. 775-784
Publication Year
1998
Abstract
The results can be concluded that the pSK 001 contains a gene for β-gal from Str. thermophilus SKD 1006, and expressed in E. coli JM 109. A 4.2 Kb of Hpa II-Hpa II fragment(lac Z of SKD 1006) of pSK 001 was subcloned into the corresponding site of pUC 19, and named pSK 002. A 1.2 Kb of Bgl II -Bgl II fragment of pSK 002 was ligated into the corresponding site of pUC 19, and named pSK 003. The nucleotide sequences of two portions(222bp close to 5' , 143 by close to 3' end) of Bgl II -Bgl II fragment of pSK 003 were determined by Sanger, Chen and Seeburg methods. The nucleotide sequences are identical to those of the gene for β-gal of Str. thermophilus A 054.
ISSN
I000-0108
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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