천연 폐 계면활성제 및 합성펩타이드와 인지질 혼합물을 이용한 폐 계면활성제의 제조와 표면활성의 물리적 특성에 관한 연구

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Title
천연 폐 계면활성제 및 합성펩타이드와 인지질 혼합물을 이용한 폐 계면활성제의 제조와 표면활성의 물리적 특성에 관한 연구
Author(s)
Joo Hyun Kang; Song Yub Shin; Cheol Young Maeng; Kil Lyong Kim; Chong Woo Bae; Kyung Soo Hahm
Bibliographic Citation
Journal of Korean Pediatric Society, vol. 41, no. 4, pp. 472-480
Publication Year
1998
Abstract
Purpose : In this study, natural pulmonary surfactant was extracted from bovine lung lavage and its surface activity was determined. To investigate the usefulness of synthetic peptides reconstituted with phospholipid as artificial surfactant, truncated peptides from surfactant protein (SP)-B were synthesized and restored the surface tension lowering activities when appropriately recombined with phospholipid. Methods : Crude natural surfactant(CNS) was isolated from lung lavage by centrifugation and organic solvent for the extraction of pulmonary surfactant was selected to satisfy the in vitro physical properties. Two truncated peptides derived from C-terminal end of bovine SP-B hydrophobic protein were selected and synthesized. To prepare artificial surfactant, synthetic peptides was added to the phospholipid mixture. The various surfactant mixtures were assayed for in vitro physical activity with the Wilhemly plate method and were determined by surface spreading rate, surface adsorption rate and surface tension-area diagram. Results : CNS-chloroform methanol(CM) displayed efficient surface activity compared with clinically used Surfactenⓡ but CNS-BuOH did not. The artificial surfactants containing phospholipid mixture and synthetic peptide were analyzed for their surface activities and displayed significant surfactant properties. Conclusion : 1-Butanol or CM(3:1) was used as an extraction solvent for CNS. CNS-CM showed more efficient surface activity than CNS-BuOH. Two synthetic peptides composing artificial pulmonary surfactant were designed and mixing ratio of peptide and phospholipid was established. Artificial surfactant dispalyed weaker surface activity than natural surfactant but significant surfactant activity. (J Korean Pediatr Soc 1998;41:472-480)
Keyword
Surfactant protein(SP)-BSurface tensionSynthetic peptideRespiratory distresssyndromeWilhemly plate method
ISSN
0560-3560
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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