Homology modeling of the receptor binding domain of human thrombopoietin

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dc.contributor.authorJin Soo Song-
dc.contributor.authorHeung Rok Park-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorMyeong Hee Yu-
dc.contributor.authorSeong Eon Ryu-
dc.date.accessioned2017-04-19T08:55:50Z-
dc.date.available2017-04-19T08:55:50Z-
dc.date.issued1998-
dc.identifier.issn0920-654X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4618-
dc.description.abstractPlatelet production in blood is regulated by a lineage specific humoral factor, thrombopoietin (TPO). The amino terminal domain of TPO (TPO-N) is responsible for the signal transduction mediated by the TPO receptor, c-mpl. From the predicted length of helices we found that TPO-N belongs to the long-chain subfamily of the four-helix bundle cytokine family. We built a three dimensional model of TPO-N by a comparative homology modeling procedure. The four helices of TPO-N with an up-up-down-down topology are stabilized by a tightly packed central hydrophobic core and the extended loop AB makes an additional hydrophobic core with helices B and D outside of the four helix bundle scaffold. An interpretation of the previous site directed mutageneses results in light of the model enabled us to identify two isolated receptor binding sites. The surface made of Lys 136, Lys 138 and Lys 140 in helix D, and Pro 42 and Glu 50 in loop AB forms the first receptor binding site, while the surface of Asp 8, Arg 10 and Lys14 in helix A represents the second binding site for the sequential receptor oligomerization.-
dc.publisherSpringer-
dc.titleHomology modeling of the receptor binding domain of human thrombopoietin-
dc.title.alternativeHomology modeling of the receptor binding domain of human thrombopoietin-
dc.typeArticle-
dc.citation.titleJournal of Computer-Aided Molecular Design-
dc.citation.number0-
dc.citation.endPage424-
dc.citation.startPage419-
dc.citation.volume12-
dc.contributor.affiliatedAuthorJin Soo Song-
dc.contributor.affiliatedAuthorHeung Rok Park-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.affiliatedAuthorMyeong Hee Yu-
dc.contributor.affiliatedAuthorSeong Eon Ryu-
dc.contributor.alternativeName송진수-
dc.contributor.alternativeName박흥록-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName유명희-
dc.contributor.alternativeName류성언-
dc.identifier.bibliographicCitationJournal of Computer-Aided Molecular Design, vol. 12, pp. 419-424-
dc.identifier.doi10.1023/A:1008064228464-
dc.subject.keywordcomparative modeling-
dc.subject.keywordcytokine-
dc.subject.keywordfour-helix bundle-
dc.subject.keywordplatelet-
dc.subject.keywordreceptor-ligand interaction-
dc.subject.localcomparative modeling-
dc.subject.localcytokine-
dc.subject.localCytokines-
dc.subject.localCytokine-
dc.subject.localfour-helix bundle-
dc.subject.localPlatelet-
dc.subject.localplatelet-
dc.subject.localplatelets-
dc.subject.localreceptor-ligand interaction-
dc.description.journalClassY-
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