Characterization of the structural proteins of hepatitis C virus expressed by an adenovirus recombinant

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Title
Characterization of the structural proteins of hepatitis C virus expressed by an adenovirus recombinant
Author(s)
Young Rim Seong; Chan Hee Lee; Dong Soo Im
Bibliographic Citation
Virus Research, vol. 55, pp. 177-185
Publication Year
1998
Abstract
Human adenoviruses have been used for mammalian expression vectors and recombinant vaccines for heterologous antigens. We constructed and characterized an infectious adenovirus recombinant containing core-E1-E2 genes of hepatitis C virus (HCV). The core protein was produced mainly during the early phase of viral infection. Expression of HCV E1 and E2 envelope proteins was detected by an immunoprecipitation with HCV-positive patient's sera. The purified E1 and E2 proteins appeared to be composed of mainly a heterodimeric form via noncovalent interaction, as previously observed in other mammalian expression systems. A small portion of E1 and E2 monomers as well as E1E2 aggregates by interdisulfide linkage were detected. Apparently heterodimeric E1E2 complexes were serologically reactive. The results suggest that adenovirus is an useful HCV antigen-expression vector.
Keyword
Adenovirus vectorHCVCoreE1E2
ISSN
0168-1702
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0168-1702(98)00043-4
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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