Biotransformation of uridine monophosphate(UMP) and glucose to uridine diphosphate-glucose(UDPG) by Candida saitoana KCTC7249 cells

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Title
Biotransformation of uridine monophosphate(UMP) and glucose to uridine diphosphate-glucose(UDPG) by Candida saitoana KCTC7249 cells
Author(s)
Jung Hun Koh; H S Shin; Y S Kim; Dae Sil Lee; C H Kim
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 60, no. 1, pp. 41-48
Publication Year
1996
Abstract
The present study investigates the biotransformation of glucose with undine monophosphate (UMP) to obtain sugar nucleotide, UDP-glucose (UDPG), by the dried cells of Candida saitoana KCTC7249. The biotransformation was optimized by varying the concentrations of substrates and phosphate ion. UDPG (24 mM) was biotransformed from 200 mM glucose and 37.5 mM UMP by dried cells of C. saitoana. The glucose yields about 64% UDP-glucose, based on UMP concentration. The addition of glucose-1-phosphate to the reaction mixture accelerated the formation of UDPG from a concentration of UMP. The structure of UDP-glucose obtained was determined with 13C NMR and FAB mass spectra. These results indicate that the yeast-dried cells could be used for the production of nucleotide sugars for donor molecules of complex carbohydrate synthesis.
Keyword
Uridine diphosphate-glucosebiotransformationC. saitoana
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02788058
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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