Design and cloning of the gene for a novel insulin analogue,(B30-homoserine) human insulin

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dc.contributor.authorD H Nam-
dc.contributor.authorJung Hun Koh-
dc.contributor.authorS Y Lee-
dc.date.accessioned2017-04-19T08:55:55Z-
dc.date.available2017-04-19T08:55:55Z-
dc.date.issued1993-
dc.identifier.issn0253-6269-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4661-
dc.description.abstractIn order to prepare a novel human insulin analogue substituted with homoserine at B30 position, (B30-homoserine) human insulin, a synthetic gene was designed by linking directly a gene for B chain with that for A chain. This gene was constructed by enzymatic joining of 10 different synthetic oligonucleotides, and then inserted at the polylinker region of pUC19 plasmid. To achieve a high level of gene expression, the gene fusion technique was employed using amino terminal regions of lacZ gene up to ClaI or Hpal, and either of them has been located under tac promoter. The chemical induction of these fused genes by isopropyl-β-D-thiogalactopyranoside (IPTG) gave a satisfactory level of expression in Escherichia coli harboring the constructed plasmids. It was observed that the fused gene product as a single chain insulin precursor was produced more than 30% of total cell protein of E. coli as a form of inclusion body.-
dc.publisherPharmaceutical Soc Korea-
dc.titleDesign and cloning of the gene for a novel insulin analogue,(B30-homoserine) human insulin-
dc.title.alternativeDesign and cloning of the gene for a novel insulin analogue,(B30-homoserine) human insulin-
dc.typeArticle-
dc.citation.titleArchives of Pharmacal Research-
dc.citation.number4-
dc.citation.endPage275-
dc.citation.startPage271-
dc.citation.volume16-
dc.contributor.affiliatedAuthorJung Hun Koh-
dc.contributor.alternativeName남두-
dc.contributor.alternativeName고정헌-
dc.contributor.alternativeName이승엽-
dc.identifier.bibliographicCitationArchives of Pharmacal Research, vol. 16, no. 4, pp. 271-275-
dc.identifier.doi10.1007/BF02977515-
dc.subject.keywordrecombinant human insulin-
dc.subject.keywordinsulin analogue-
dc.subject.keywordinsulin [b30 homoserine]-
dc.subject.keywordsingle chain insulin precursor-
dc.subject.keywordgene fusion-
dc.subject.localrecombinant human insulin-
dc.subject.localinsulin analogue-
dc.subject.localInsulin analogue-
dc.subject.localinsulin [b30 homoserine]-
dc.subject.localsingle chain insulin precursor-
dc.subject.localSingle chain insulin precursor-
dc.subject.localgene fusion-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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