Use of high culture pH for the decreased proteolysis of human parathylroid hormone secreted by Saccharomyces cerevisiae

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Title
Use of high culture pH for the decreased proteolysis of human parathylroid hormone secreted by Saccharomyces cerevisiae
Author(s)
Gu Yong Song; Bong Hyun Chung
Bibliographic Citation
Biotechnology Letters, vol. 21, pp. 57-60
Publication Year
1999
Abstract
Human parathyroid hormone (hPTH) was expressed and secreted in Saccharomyces cerevisiae. In batch fermentations performed at pH = 5.6, 6.5, 7.2 and 7.5, optimal production of hPTH (12.1 mg/l) was obtained at pH 7.2 after 24 h of culture. At pH 5.6, most of secreted hPTH was degraded. Proteolysis of hPTH was significantly decreased by increasing the culture pH.
Keyword
human parathyroid hormoneculture pHproteolysisSaccharomyces cerevisiae
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005410222692
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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