DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo Kyeong Kim | - |
dc.contributor.author | Dae Yeul Yu | - |
dc.contributor.author | Kang Hyun Ah | - |
dc.contributor.author | Sun Yoon | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.date.accessioned | 2017-04-19T08:55:56Z | - |
dc.date.available | 2017-04-19T08:55:56Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4664 | - |
dc.description.abstract | A recombinant human α(s1)-casein was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase, and human α(s1)- casein were used to direct the secretion of human α(s1)-casein into the extracellular medium. Among the three leader sequences tested, the native leader sequence of human α(s1)-casein was found to be the most efficient in the secretory expression of human α(s1)-casein, which implies that the native leader sequence of human α(s1)-casein might be used very efficiently for the secretory production of other heterologous proteins in yeast. The recombinant human α(s1)-casein was proteolytically cleaved as the culture proceeded. Therefore, an attempt was made to produce human α(s1)-casein using a S. cerevisiae mutant in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 72 h of culture, most of the human α(s1)-casein secreted by the wild type was cleaved, whereas more than 70% of the human α(s1)-casein secreted by yap3-disruptant remained intact. The results suggest that YAP3 might be involved in the internal cleavage of human α(s1)-casein expressed in yeast. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Secretory expression of human αs₁-casein in Saccharomyces cerevisiae | - |
dc.title.alternative | Secretory expression of human αs₁-casein in Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 200 | - |
dc.citation.startPage | 196 | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Yoo Kyeong Kim | - |
dc.contributor.affiliatedAuthor | Dae Yeul Yu | - |
dc.contributor.affiliatedAuthor | Kang Hyun Ah | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 김유경 | - |
dc.contributor.alternativeName | 유대열 | - |
dc.contributor.alternativeName | 강현아 | - |
dc.contributor.alternativeName | 윤선 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 196-200 | - |
dc.subject.keyword | Human α(s1)-casein | - |
dc.subject.keyword | secretion | - |
dc.subject.keyword | leader sequence | - |
dc.subject.keyword | proteolysis | - |
dc.subject.keyword | Saccharomyces cerevisiae | - |
dc.subject.local | Human α(s1)-casein | - |
dc.subject.local | human α(s1)-casein | - |
dc.subject.local | Secretion | - |
dc.subject.local | secretion | - |
dc.subject.local | leader sequence | - |
dc.subject.local | Proteolysis | - |
dc.subject.local | proteolysis | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.description.journalClass | Y | - |
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