Secretory expression of human αs₁-casein in Saccharomyces cerevisiae

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dc.contributor.authorYoo Kyeong Kim-
dc.contributor.authorDae Yeul Yu-
dc.contributor.authorKang Hyun Ah-
dc.contributor.authorSun Yoon-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T08:55:56Z-
dc.date.available2017-04-19T08:55:56Z-
dc.date.issued1999-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4664-
dc.description.abstractA recombinant human α(s1)-casein was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase, and human α(s1)- casein were used to direct the secretion of human α(s1)-casein into the extracellular medium. Among the three leader sequences tested, the native leader sequence of human α(s1)-casein was found to be the most efficient in the secretory expression of human α(s1)-casein, which implies that the native leader sequence of human α(s1)-casein might be used very efficiently for the secretory production of other heterologous proteins in yeast. The recombinant human α(s1)-casein was proteolytically cleaved as the culture proceeded. Therefore, an attempt was made to produce human α(s1)-casein using a S. cerevisiae mutant in which the YAP3 gene encoding yeast aspartic protease 3 (YAP3) was disrupted. After 72 h of culture, most of the human α(s1)-casein secreted by the wild type was cleaved, whereas more than 70% of the human α(s1)-casein secreted by yap3-disruptant remained intact. The results suggest that YAP3 might be involved in the internal cleavage of human α(s1)-casein expressed in yeast.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleSecretory expression of human αs₁-casein in Saccharomyces cerevisiae-
dc.title.alternativeSecretory expression of human αs₁-casein in Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number2-
dc.citation.endPage200-
dc.citation.startPage196-
dc.citation.volume9-
dc.contributor.affiliatedAuthorYoo Kyeong Kim-
dc.contributor.affiliatedAuthorDae Yeul Yu-
dc.contributor.affiliatedAuthorKang Hyun Ah-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName김유경-
dc.contributor.alternativeName유대열-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName윤선-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 9, no. 2, pp. 196-200-
dc.subject.keywordHuman α(s1)-casein-
dc.subject.keywordsecretion-
dc.subject.keywordleader sequence-
dc.subject.keywordproteolysis-
dc.subject.keywordSaccharomyces cerevisiae-
dc.subject.localHuman α(s1)-casein-
dc.subject.localhuman α(s1)-casein-
dc.subject.localSecretion-
dc.subject.localsecretion-
dc.subject.localleader sequence-
dc.subject.localProteolysis-
dc.subject.localproteolysis-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localsaccharomyces cerevisiae-
dc.description.journalClassY-
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