수종의 국화과 식물에서 분리한 Sesquiterpene lactone들의 생리활성(제3보). Nitric oxide 방출 및 ACAT 저해활성

Abstract

Nine sesquiterpene lactones, which were isolated from Hemisteptia lyrata Bunge, Chrysanthemum zawadskii Herbich var. latilobum Kitamura and Chrysanthemum boreale Makino were evaluated for the inhibition upon the nitric oxide release from the Raw cell and for the ACAT (acyl Co A: cholesterol acyltransferase) inhibitory assay. In the nitric oxide release inhibitory experiment, hemistepsin B (IC_(50)=0.05 ㎍/㎖, 0.15 μM), cumambrin B, tulipinolide and costunolide exhibited strong inhibition with IC_(50) values 0.05 ㎍/㎖ (0.15 μM), 0.1 ㎍/㎖ (0.38 μM), 0.2 ㎍/㎖ (0.69 μM) and 0.2 ㎍/㎖ (0.86 μM), respectively. In the ACAT inhibitory assay, angeloylcumambrin B, tigloylcumambrin B, tulipinolide and costunolide exhibited strong inhibition with IC_(50) values 33 ㎍/㎖ (95.4 μM), 22 ㎍/㎖ (63.6 μM), 38 ㎍/㎖ (151 μM) and 17 ㎍/㎖ (73.3 μM), respectively, whereas other compounds did not exhibit significant activity against ACAT above 100 ㎍/㎖.