Vectors for rapid selection of intergrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1

Abstract

Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DLI. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in law (1-2), moderate (6-9) or high (up to 100) copy numbers. The vectors and mutants described are potentially useful for the construction of efficient producers of heterologous proteins in N. polymorpha.