Screening and taxonomic characterization of D-amino acid aminotransferase-producing thermophiles = D-amino acid aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

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Title
Screening and taxonomic characterization of D-amino acid aminotransferase-producing thermophiles = D-amino acid aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구
Author(s)
Mi Sun Kwak; Seung Goo Lee; Sang Cheol Jeong; Seung Hyun Suh; Jae Heung Lee; Yeong Joong Jeon; Young Ho Kim; Moon Hee Sung
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 27, no. 3, pp. 184-190
Publication Year
1999
Abstract
To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (DAAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. LK-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.
Keyword
screeningD-amino acid aminotransferasetaxonomic characterizationthermophiles
ISSN
0257-2389
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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