Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain

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Title
Molecular cloning of multiple splicing variants of JIP-1 preferentially expressed in brain
Author(s)
In Jung Kim; Ko Woon Lee; Byung Young Park; Ja Kyeong Lee; Ji Hyun Park; In Young Choi; Soo Jung Eom; Tong Shin Chang; Myung Jin Kim; Young Il Yeom; Sung Key Chang; Young Don Lee; Eui Ju Choi; Pyung Lim Han
Bibliographic Citation
Journal of Neurochemistry, vol. 72, no. 4, pp. 1335-1343
Publication Year
1999
Abstract
Stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) is activated by a variety of cellular or environmental stresses. Proper regulation of the SAPK/JNK pathway may be critical for cell survival or death under various conditions. In this study, we report the molecular cloning of novel isoforms of JIP-1, which harbor a putative phosphotyrosine interaction domain and a helix-loop-helix domain, as well as an SH3 homologous region in the C terminus. Northern analysis indicates that transcription variant jip-1 is expressed in brain and kidney and transcription variants jip-2 and jip-3 are specifically expressed in brain. In situ hybridization data showed that the hybridized jip messages were heavily concentrated in adult brain, and were particularly enriched in the cerebral cortex and hippocampus, the brain regions vulnerable to pathological states such as hypoxia-ischemia, epilepsy, and Alzheimer's disease. All the deduced protein products of the jip transcription variants appear to have a similar property in that they inhibit the SAPK/JNK stimulation when overexpressed. Inhibition of SAPK activation by overexpression of the novel isoform JIP-2a resulted in suppression of etoposide-induced cell death in a neuroglioma cell line, N18TG. These findings suggest that JIP may play an important role in regulation of the SAPK pathway that is involved in stress-induced cellular responses.
Keyword
SAPK/JNKApoptosisAnti-cell death functionBrain expressionJIP isoforms
ISSN
0022-3042
Publisher
Wiley
DOI
http://dx.doi.org/10.1046/j.1471-4159.1999.721335.x
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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