Transcriptional analysis of levU operon encoding saccharolytic enzymes and two apparent genes involved in amino acid biosynthesis in Zymomonas mobilis

Cited 16 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKi Bang Song-
dc.contributor.authorJeong Woo Seo-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:56:02Z-
dc.date.available2017-04-19T08:56:02Z-
dc.date.issued1999-
dc.identifier.issn03781119-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4705-
dc.description.abstractExtracellular levansucrase (LevU) and sucrase (InvB) are two of the three saccharolytic enzymes involved in the sucrose metabolism of Zymomonas mobilis. The levU and invB genes were clustered with a 155 bp interval on the chromosome. Both genes were transcribed constitutively at the basal level and the transcription of both genes was induced significantly when sucrose was added to the medium. These genes were transcribed as a bicistronic mRNA and the expression was modulated by a single promoter, which is located upstream of the levU gene. The transcriptional initiation site was mapped to -64 bp from the translation start site of levU gene. These results indicated that two genes are most likely to constitute an operon. The glk operon, which encodes four glycolytic enzymes, was located close to the levU operon on the chromosome. Two apparent ORFs (ORF3 and 4) were found at the intervening sequence located between the glk and levU operons. These ORFs were transcribed divergently and showed high homology at the amino acid level with the bacterial global regulatory protein (Lrp) and aspartate racemase.-
dc.publisherElsevier-
dc.titleTranscriptional analysis of levU operon encoding saccharolytic enzymes and two apparent genes involved in amino acid biosynthesis in Zymomonas mobilis-
dc.title.alternativeTranscriptional analysis of levU operon encoding saccharolytic enzymes and two apparent genes involved in amino acid biosynthesis in Zymomonas mobilis-
dc.typeArticle-
dc.citation.titleGene-
dc.citation.number0-
dc.citation.endPage114-
dc.citation.startPage107-
dc.citation.volume232-
dc.contributor.affiliatedAuthorJeong Woo Seo-
dc.contributor.alternativeName송기방-
dc.contributor.alternativeName서정우-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationGene, vol. 232, pp. 107-114-
dc.identifier.doi10.1016/S0378-1119(99)00106-7-
dc.subject.keywordAspartate racemase-
dc.subject.keywordLevansucrase-
dc.subject.keywordLrp-like protein-
dc.subject.keywordSucrase-
dc.subject.keywordTranscription-
dc.subject.localAspartate racemase-
dc.subject.localLevansucrase-
dc.subject.localLrp-like protein-
dc.subject.localSucrase-
dc.subject.localTranscription-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.