Cited 0 time in
- Molecular cloning and analysis of sporulation-specific glucoamylase(SGA) gene of Saccharomyces diastaticus
- Dae Ook Kang; In Kyu Hwang; Won Keun Oh; Hyun Sun Lee; Soon Cheol Ahn; Bo Yeon Kim; Tae Ick Mheen; Jong Seog Ahn
- Bibliographic Citation
- Journal of Microbiology, vol. 37, no. 1, pp. 35-40
- Publication Year
- Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.
- S. DiastaticusSGA gene cloninggene disruptionsecretory protein
- Microbiological Society Korea
- Appears in Collections:
- Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.