Molecular cloning and analysis of sporulation-specific glucoamylase(SGA) gene of Saccharomyces diastaticus

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Title
Molecular cloning and analysis of sporulation-specific glucoamylase(SGA) gene of Saccharomyces diastaticus
Author(s)
Dae Ook Kang; In Kyu Hwang; Won Keun Oh; Hyun Sun Lee; Soon Cheol Ahn; Bo Yeon KimTae Ick MheenJong Seog Ahn
Bibliographic Citation
Journal of Microbiology, vol. 37, no. 1, pp. 35-40
Publication Year
1999
Abstract
Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.
Keyword
S. DiastaticusSGA gene cloninggene disruptionsecretory protein
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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