DC Field | Value | Language |
---|---|---|
dc.contributor.author | Won Kyung Kim | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.date.accessioned | 2017-04-19T08:56:03Z | - |
dc.date.available | 2017-04-19T08:56:03Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4711 | - |
dc.description.abstract | The high density mixed mode adsorbent known by the trade name of Mimo- AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to >95% with an overall recovery yield of 66%. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Direct purification of lysozyme from hen egg white using high density mixed mode adsorbent | - |
dc.title.alternative | Direct purification of lysozyme from hen egg white using high density mixed mode adsorbent | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 296 | - |
dc.citation.startPage | 292 | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Won Kyung Kim | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 김원경 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 9, no. 3, pp. 292-296 | - |
dc.subject.keyword | Lysozyme | - |
dc.subject.keyword | hen egg white (HEW) | - |
dc.subject.keyword | mixed mode adsorbent | - |
dc.subject.keyword | expanded bed | - |
dc.subject.local | lysozyme | - |
dc.subject.local | Lysozyme | - |
dc.subject.local | hen egg white (HEW) | - |
dc.subject.local | mixed mode adsorbent | - |
dc.subject.local | expanded bed | - |
dc.description.journalClass | Y | - |
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