Direct purification of lysozyme from hen egg white using high density mixed mode adsorbent

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dc.contributor.authorWon Kyung Kim-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T08:56:03Z-
dc.date.available2017-04-19T08:56:03Z-
dc.date.issued1999-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4711-
dc.description.abstractThe high density mixed mode adsorbent known by the trade name of Mimo- AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to >95% with an overall recovery yield of 66%. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDirect purification of lysozyme from hen egg white using high density mixed mode adsorbent-
dc.title.alternativeDirect purification of lysozyme from hen egg white using high density mixed mode adsorbent-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number3-
dc.citation.endPage296-
dc.citation.startPage292-
dc.citation.volume9-
dc.contributor.affiliatedAuthorWon Kyung Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName김원경-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 9, no. 3, pp. 292-296-
dc.subject.keywordLysozyme-
dc.subject.keywordhen egg white (HEW)-
dc.subject.keywordmixed mode adsorbent-
dc.subject.keywordexpanded bed-
dc.subject.locallysozyme-
dc.subject.localLysozyme-
dc.subject.localhen egg white (HEW)-
dc.subject.localmixed mode adsorbent-
dc.subject.localexpanded bed-
dc.description.journalClassY-
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