Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes

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Title
Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes
Author(s)
Tae Hoon Lee; Seong Lan Yu; Sun-Uk Kim; Yong Man Kim; In Pyo Choi; Sang Won Kang; Sue Goo Rhee; Dae Yeul Yu
Bibliographic Citation
Gene, vol. 234, pp. 337-344
Publication Year
1999
Abstract
A new type of peroxiredoxin, named 1-Cys peroxiredoxin (1-Cys Prx), reduces hydrogen peroxide with the use of electrons from unidentified electron donor(s). We have isolated the mouse gene encoding 1-Cys Prx (CP-3) and shown that it is comprised of five exons and four introns. Analysis of 5' flanking regions revealed binding sequences of several putative transcription factors such as Sp1, Pit-1a, c-Jun, c-Myc and YY1. It is noticeable that several potential Sp1 binding sites assigned the -60 through -96 bp from putative transcription initiation site. The gel shift assays showed that Sp1 and Pit-1a bind specifically to each binding site in 1-Cys Prx promoter. We also isolated two highly related genes such as CP-2 and CP-5. These genes are encoded by single exons, and show 85% of nucleotide sequence homology with the CP-3. The structural features of these genes suggest that they might be intronless genes derived from the CP-3 by the mechanism involving retrotransposition. In addition, our data suggest that they are inserted to a specific site of the mouse L1 repetitive element. The 1-Cys Prx was actively transcribed in a variety of adult tissues as well as in the developing embryos. These results suggest that only the 1-Cys Prx gene might be relevant for studying the function of the 1-Cys Prx in the murine system.
Keyword
Antioxidant enzymeBacterial artificial chromosomeFamily geneRetrotransposition
ISSN
0378-1119
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0378-1119(99)00190-0
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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