Suppression of proteolytic degradation of recombinant hirudin from Saccharomyces cerevisiae using the O₂-enriched air

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Title
Suppression of proteolytic degradation of recombinant hirudin from Saccharomyces cerevisiae using the O₂-enriched air
Author(s)
K Jagannadha Rao; Chul Ho Kim; Bong Hyun Chung; Myung Kuk Kim; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 21, no. 5, pp. 391-394
Publication Year
1999
Abstract
A novel process using O2-enriched air supply was used to suppress the C-terminal proteolytic degradation of recombinant hirudin (r-hirudin) from Saccharomyces cerevisiae. When dissolved O2 was controlled above 20% saturation level using normal air, inactive forms of C-terminally truncated hirudin were observed in culture broth from 48 h of fermentation. The use of O2-enriched air giving above 40% saturation of dissolved O2 suppressed the proteolytic degradation and hence the formation of truncated forms of inactive r-hirudin until 60 h of fermentation.
Keyword
C-terminally truncated hirudinproteolytic degradationrecombinant hirudin
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005443306866
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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