Analysis of low-level α-D-glucose-1-phosphate in thermophilic enzyme reaction mixuture using high pH anion-exchange chromatograph = 고성능 액체 크로마토그래프를 이용한 내열성 효소반응 산물인 α-D-glucose-1-phosphate의 저농도 분석

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHyun Jae Shin-
dc.contributor.authorYoung Sook Shin-
dc.contributor.authorDae Sil Lee-
dc.date.accessioned2017-04-19T08:56:06Z-
dc.date.available2017-04-19T08:56:06Z-
dc.date.issued1999-
dc.identifier.issn1225-7117-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4731-
dc.description.abstractWe have used high pH anion-exchange chromatography to analyze liw level (below 20μM) α-ㅇ-glucose-1-phosphate (g-1-P) that can be used as a cytostatic compound, an antibiotic, and immunosuppressive drug. Our chromatographic method afforded excellemt peak resolution and seletivith fro glucose-6-phosphate and various maltooligosaccharides as well as G-1-P. The pulsed amperometric detector yielded linear response on G-1-P ranging from 2 - 20 μM, giving slope of 4.8X10⁴ (peak area/μM). The detection limit was 2 μM. This method was applied to the purification of thermophilic α-glucan phosphorylase from Thermus caldophilus. The technique will be extremely useful in future studies concerning carbohydrate metabolism in living organisms.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleAnalysis of low-level α-D-glucose-1-phosphate in thermophilic enzyme reaction mixuture using high pH anion-exchange chromatograph = 고성능 액체 크로마토그래프를 이용한 내열성 효소반응 산물인 α-D-glucose-1-phosphate의 저농도 분석-
dc.title.alternativeAnalysis of low-level α-D-glucose-1-phosphate in thermophilic enzyme reaction mixuture using high pH anion-exchange chromatograph-
dc.typeArticle-
dc.citation.titleKorean Journal of Biotechnology and Bioengineering-
dc.citation.number3-
dc.citation.endPage388-
dc.citation.startPage384-
dc.citation.volume14-
dc.contributor.affiliatedAuthorHyun Jae Shin-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName신현재-
dc.contributor.alternativeName신영숙-
dc.contributor.alternativeName이대실-
dc.identifier.bibliographicCitationKorean Journal of Biotechnology and Bioengineering, vol. 14, no. 3, pp. 384-388-
dc.description.journalClassN-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.