Isolation and charaterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase

Cited 0 time in scopus
Metadata Downloads
Title
Isolation and charaterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase
Author(s)
Mi Hui Hong; Yong Hee Lee; Jae Wha Kim; Jong-Seok Lim; Sung Yeoul Chang; Kwang Soo Lee; Sang Gi Paik; In Seong Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 47, no. 3, pp. 407-415
Publication Year
1999
Abstract
A cDNA clone encoding 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) was isolated from a human fetal liver cDNA library. It contained the open reading frame of 2709 base pairs and predicted a protein comprising 902 amino acids with a calculated molecular weight of 98,700 Da. The deduced protein showed about 93.6% homology (90.5% identity, 3.1% favored substitutions) when compared with rat 10-FTHFDH. The distribution of 10-FTHFDH transcript in various human tissues was studied by Northern blot analysis using poly(A+) RNAs from different tissues. The 10-FTHFDH transcript with an approximate size of 2.7 kb was mainly expressed in human kidney, skeletal muscle, and liver and rarely expressed in other tissues.
Keyword
10-formyltetrahydrofolate dehydrogenase (10-FTHFDH)cDNAtissue distributionhuman
ISSN
1039-9712
Publisher
Wiley
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.