Cloning of the genomic sequence encoding a processed adenylate kinase 2 pseudogene

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Title
Cloning of the genomic sequence encoding a processed adenylate kinase 2 pseudogene
Author(s)
Yong Hee Lee; Mi Hui Hong; Yeon Jung Kim; Jae Wha Kim; Cheorl Ho Kim; Kwang Soo Lee; Sung Yeoul Chang; Jong-Seok Lim; In Seong Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 47, no. 1, pp. 37-46
Publication Year
1999
Abstract
A chromosomal DNA sequence harboring a processed AK2B pseudogene was isolated from a human genomic library. It was a variant of the AK2B gene sequence including several point mutations, deletions, and insertions. The nucleotide sequence of the ORF of the AK2B pseudogene predicted a truncated form of the AK2B mutant suggesting that the processed pseudogene is nonfunctional. A repetitive sequence, AAAAGAGAG, found in the 5' and 3' flanking regions of the pseudogene and the poly(A) tract in the 3' end junction suggest that a mRNA of AK2B may have been converted to the processed pseudogene by retrotransposition events. Previously, it was suggested that an adenylate kinase (AK) 2 related gene on chromosome 2, confirmed by Southern analysis using somatic cell hybrid cell lines, may be a processed pseudogene. It is proposed that the processed pseudogene isolated in this study may be the AK2 related nonfunctional gene localized on human chromosomes 2.
Keyword
genomic sequenceAK2 processed pseudogenechromosomal locus
ISSN
1039-9712
Publisher
Wiley
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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