Production and characterization of an antibody specific for a novel protein serine/Threonine kinase, MPK38, highly expressed in hematopoietic cells

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dc.contributor.authorYoung Yang-
dc.contributor.authorMin Chan Gil-
dc.contributor.authorYoung Hoon Lee-
dc.contributor.authorHyun Jung Ha-
dc.date.accessioned2017-04-19T08:56:13Z-
dc.date.available2017-04-19T08:56:13Z-
dc.date.issued1999-
dc.identifier.issn0273-2289-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4764-
dc.description.abstractWe report an antibody that selectively recognizes MPK38, a new protein serine/threonine kinase closely related to the SNF1 serine/threonine kinase family. This antibody recognized a region of the N-terminal kinase catalytic domain and part of the remaining C-terminal portion and was sensitive enough to detect a 72-kDa recombinant MPK38 in insect cells by Western blotting. Immunoblot analysis showed that the recombinant MPK38 was expressed in a time-dependent manner and reached a maximum after 48 h postinfection. In addition, the immune complex kinase assay revealed that the recombinant and endogenous MPK38 protein autophosphorylated in vitro. Phosphoamino acid analysis of autophosphorylated MPK38 protein showed that the phosphorylation was exclusively on serine and threonine residues, suggesting that MPK38 is a protein serine/threonine kinase. Thus, this antibody could be helpful for elucidating the biological functions of MPK38 in the MPK38-expressing cells.-
dc.publisherSpringer-
dc.titleProduction and characterization of an antibody specific for a novel protein serine/Threonine kinase, MPK38, highly expressed in hematopoietic cells-
dc.title.alternativeProduction and characterization of an antibody specific for a novel protein serine/Threonine kinase, MPK38, highly expressed in hematopoietic cells-
dc.typeArticle-
dc.citation.titleApplied Biochemistry and Biotechnology-
dc.citation.number0-
dc.citation.endPage22-
dc.citation.startPage13-
dc.citation.volume80-
dc.contributor.affiliatedAuthorYoung Yang-
dc.contributor.affiliatedAuthorMin Chan Gil-
dc.contributor.alternativeName양영-
dc.contributor.alternativeName길민찬-
dc.contributor.alternativeName이영훈-
dc.contributor.alternativeName하현정-
dc.identifier.bibliographicCitationApplied Biochemistry and Biotechnology, vol. 80, pp. 13-22-
dc.identifier.doi10.1385/ABAB:80:1:13-
dc.subject.keywordAnti-MPK38 antibody-
dc.subject.keywordserine/threonine kinase-
dc.subject.keywordautophosphorylation-
dc.subject.keywordsignal transduction-
dc.subject.keywordrecombinant protein-
dc.subject.localAnti-MPK38 antibody-
dc.subject.localserine/threonine kinase-
dc.subject.localAutophosphorylation-
dc.subject.localautophosphorylation-
dc.subject.localSignal transduction-
dc.subject.localSignal trasduction-
dc.subject.localsignal transduction-
dc.subject.localRecombinant Protein-
dc.subject.localrecombinant protein-
dc.subject.localrecombinant proteins-
dc.subject.localRecombinant protein-
dc.subject.localecombinant proteins-
dc.subject.localRecombinant proteins-
dc.description.journalClassY-
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