DC Field | Value | Language |
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dc.contributor.author | Jae W Bang | - |
dc.contributor.author | Dong S In | - |
dc.contributor.author | Sung H Chung | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.date.accessioned | 2017-04-19T08:56:14Z | - |
dc.date.available | 2017-04-19T08:56:14Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0167-6857 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4770 | - |
dc.description.abstract | Hypocotyl segments of Bupleurum falcatum L. formed embryogenic calluses when cultured on Murashige and Skoog's (MS) medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Suspension cultures were initiated by placing calluses into medium with 0.45 μM 2,4-D. Protoplasts were enzymatically isolated from suspension cultures. They were plated at a density of 5 x 104 protoplasts per ml on MS medium supplemented with 9% mannitol, 9.0 μM 2,4-D, 4.4 μM BA, 4.6 μM kinetin, and 0.6% Seaplaque agarose. After four weeks of culture, microcalluses were formed and subsequently transferred to MS solid medium with 18.1 μM 2,4-D. Upon transfer to MS basal medium, microcalluses gave rise to somatic embryos at a frequency of approximately 10%. They subsequently developed into plantlets. The regenerants were successfully transplanted to potting soil and grown to maturity in a greenhouse. The regenerants had the normal chromosome number of 2n=2x=20 and did not show morphological aberrancy. | - |
dc.publisher | Springer | - |
dc.title | Plant regeneration from embryogenic cells-derived protoplasts of Bupleurum falcatum | - |
dc.title.alternative | Plant regeneration from embryogenic cells-derived protoplasts of Bupleurum falcatum | - |
dc.type | Article | - |
dc.citation.title | Plant Cell Tissue and Organ Culture | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 154 | - |
dc.citation.startPage | 151 | - |
dc.citation.volume | 55 | - |
dc.contributor.affiliatedAuthor | Dong S In | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.alternativeName | 방재욱 | - |
dc.contributor.alternativeName | 인동수 | - |
dc.contributor.alternativeName | 정성현 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.identifier.bibliographicCitation | Plant Cell Tissue and Organ Culture, vol. 55, no. 2, pp. 151-154 | - |
dc.identifier.doi | 10.1023/A:1006257122561 | - |
dc.subject.keyword | protoplast | - |
dc.subject.keyword | somaclonal variation | - |
dc.subject.keyword | somatic embryo | - |
dc.subject.keyword | tissue culture | - |
dc.subject.keyword | Umbelliferae | - |
dc.subject.local | Protoplast | - |
dc.subject.local | protoplast | - |
dc.subject.local | protoplasts | - |
dc.subject.local | somaclonal variation | - |
dc.subject.local | Somatic embryo | - |
dc.subject.local | somatic embryo | - |
dc.subject.local | somatic embryos | - |
dc.subject.local | Tissue culture | - |
dc.subject.local | tissue culture | - |
dc.subject.local | Umbelliferae | - |
dc.subject.local | umbeliferae | - |
dc.subject.local | umbelliferae | - |
dc.description.journalClass | Y | - |
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