Cited 8 time in
- Title
- Synthesis of alkaline protease by catabolite repression-resistant Thermoactinomyces sp. E79 mutant
- Author(s)
- K Sunitha; Young Seo Park; Tae Kwang Oh; Jung Kee Lee
- Bibliographic Citation
- Biotechnology Letters, vol. 21, pp. 155-158
- Publication Year
- 1999
- Abstract
- The production of alkaline protease from Thermoactinomyces sp. E79 was repressed by 0.2% (w/v) glucose in the medium. Catabolite repression-resistant mutant M1 was obtained by combined treatment of UV light and N-methyl-N'-nitro-N-nitrosoguanidine. The glucose uptake studies accomplished by [14C]glucose showed that the mutant has lost its ability for glucose uptake. The protease production by mutant M1 in the enzyme production medium was 62 U/mg, which was twice that of the wild-type strain.
- Keyword
- catabolite repressionmutationglucose transportproteaseThermoactinomyces
- ISSN
- 0141-5492
- Publisher
- Springer
- DOI
- http://dx.doi.org/10.1023/A:1005479508156
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.