Minor thermodtable alkaline protease produced by Thermoactinomyces sp. E79

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dc.contributor.authorYoung Ok Kim-
dc.contributor.authorJung Kee Lee-
dc.contributor.authorKandula Sunitha-
dc.contributor.authorHyung Kwoun Kim-
dc.contributor.authorTae Kwang Oh-
dc.date.accessioned2017-04-19T08:56:15Z-
dc.date.available2017-04-19T08:56:15Z-
dc.date.issued1999-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4777-
dc.description.abstractThermoactinomyces sp. E79 produced two types of thermostable alkaline proteases extracellularly. A minor protease was separated from a major protease by using DEAE-column chromatography. This enzyme was purified to homogeneity by ammonium sulfate and DEAE-Sepharose ion-exchange chromatography. The purified minor protease showed different biochemical properties compared to the major protease. The molecular mass of the purified enzyme was estimated by SDS-PAGE to be 36 kDa. Its optimum temperature and pH for proteolytic activity against Hammarsten casein were 70°C and 9.0, respectively. The enzyme was stable up to 75°C and in an alkaline pH range of 9.0-11.0. The enzyme was inhibited by phenylmethylsulfonyl fluoride (PMSF) and Hg2+, indicating that the enzyme may be a cysteine-dependent serine protease. In addition, the enzyme cleaved the endoproteinase substrate, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, and the K(m) value for the substrate was 1.2 mM.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMinor thermodtable alkaline protease produced by Thermoactinomyces sp. E79-
dc.title.alternativeMinor thermodtable alkaline protease produced by Thermoactinomyces sp. E79-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number4-
dc.citation.endPage474-
dc.citation.startPage469-
dc.citation.volume9-
dc.contributor.affiliatedAuthorYoung Ok Kim-
dc.contributor.affiliatedAuthorJung Kee Lee-
dc.contributor.affiliatedAuthorHyung Kwoun Kim-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName김영옥-
dc.contributor.alternativeName이정기-
dc.contributor.alternativeNameSunitha-
dc.contributor.alternativeName김형권-
dc.contributor.alternativeName오태광-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 9, no. 4, pp. 469-474-
dc.subject.keywordthermoactinomyces-
dc.subject.keywordthermophile-
dc.subject.keywordalkaline protease-
dc.subject.keywordthermostable enzyme-
dc.subject.keywordpurification-
dc.subject.localthermoactinomyces-
dc.subject.localThermoactinomyces-
dc.subject.localThermophile-
dc.subject.localthermophile-
dc.subject.localThermophiles-
dc.subject.localalkaline protease-
dc.subject.localthermostable enzyme-
dc.subject.localThermostable enzyme-
dc.subject.localPurification-
dc.subject.localpurification-
dc.subject.localPurifcation-
dc.description.journalClassY-
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Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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