Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus

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dc.contributor.authorHyun Jae Shin-
dc.contributor.authorMi Kyung Kim-
dc.contributor.authorDae Sil Lee-
dc.date.accessioned2017-04-19T08:56:18Z-
dc.date.available2017-04-19T08:56:18Z-
dc.date.issued1999-
dc.identifier.issn1389-1723-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4797-
dc.description.abstractN-Acetylglucosamine 6-phosphate deacetylase [EC 3.5.1.25] was purified and biochemically characterized from an extreme thermophile, Thermus caldophilus GK24. The optimum temperature and pH of the enzyme were 80°C and 7.5, respectively. The enzyme is a tetramer composed of identical 45 kDa subunits. The N-terminal amino acid sequence of the purified enzyme was determined to be MSVDLKTLHRRHVLTP. It hydrolyzed GlcNAc-6-P, but not GlcNAc- 1-P or chitin oligosaccharides. The deacetylase activity was completely inhibited by the addition of 1 mM Cu2+, but moderately activated by that of 1 mM Mn2+ and Co2+. Within 2 h of reaction, 2 mM GlcNAc-6-P was completely hydrolyzed to GlcN-6-P and acetate by the action of the deacetylase.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titlePurification and characterization of N-acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus-
dc.title.alternativePurification and characterization of N-acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus-
dc.typeArticle-
dc.citation.titleJournal of Bioscience and Bioengineering-
dc.citation.number3-
dc.citation.endPage322-
dc.citation.startPage319-
dc.citation.volume88-
dc.contributor.affiliatedAuthorHyun Jae Shin-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName신현재-
dc.contributor.alternativeName김미경-
dc.contributor.alternativeName이대실-
dc.identifier.bibliographicCitationJournal of Bioscience and Bioengineering, vol. 88, no. 3, pp. 319-322-
dc.identifier.doi10.1016/S1389-1723(00)80017-1-
dc.subject.keywordN-acetylglucosamine 6-phosphate deacetylase-
dc.subject.keywordThermus caldophilus-
dc.subject.keywordnagA gene-
dc.subject.localN-acetylglucosamine 6-phosphate deacetylase-
dc.subject.localthermus caldophillus-
dc.subject.localThermus caldophilus-
dc.subject.localthermus caldophilus-
dc.subject.localnagA gene-
dc.description.journalClassY-
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