DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun Jae Shin | - |
dc.contributor.author | Mi Kyung Kim | - |
dc.contributor.author | Dae Sil Lee | - |
dc.date.accessioned | 2017-04-19T08:56:18Z | - |
dc.date.available | 2017-04-19T08:56:18Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4797 | - |
dc.description.abstract | N-Acetylglucosamine 6-phosphate deacetylase [EC 3.5.1.25] was purified and biochemically characterized from an extreme thermophile, Thermus caldophilus GK24. The optimum temperature and pH of the enzyme were 80°C and 7.5, respectively. The enzyme is a tetramer composed of identical 45 kDa subunits. The N-terminal amino acid sequence of the purified enzyme was determined to be MSVDLKTLHRRHVLTP. It hydrolyzed GlcNAc-6-P, but not GlcNAc- 1-P or chitin oligosaccharides. The deacetylase activity was completely inhibited by the addition of 1 mM Cu2+, but moderately activated by that of 1 mM Mn2+ and Co2+. Within 2 h of reaction, 2 mM GlcNAc-6-P was completely hydrolyzed to GlcN-6-P and acetate by the action of the deacetylase. | - |
dc.publisher | Soc Bioscience Bioengineering Japan | - |
dc.title | Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus | - |
dc.title.alternative | Purification and characterization of N-acetylglucosamine 6-phosphate deacetylase from Thermus caldophilus | - |
dc.type | Article | - |
dc.citation.title | Journal of Bioscience and Bioengineering | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 322 | - |
dc.citation.startPage | 319 | - |
dc.citation.volume | 88 | - |
dc.contributor.affiliatedAuthor | Hyun Jae Shin | - |
dc.contributor.affiliatedAuthor | Dae Sil Lee | - |
dc.contributor.alternativeName | 신현재 | - |
dc.contributor.alternativeName | 김미경 | - |
dc.contributor.alternativeName | 이대실 | - |
dc.identifier.bibliographicCitation | Journal of Bioscience and Bioengineering, vol. 88, no. 3, pp. 319-322 | - |
dc.identifier.doi | 10.1016/S1389-1723(00)80017-1 | - |
dc.subject.keyword | N-acetylglucosamine 6-phosphate deacetylase | - |
dc.subject.keyword | Thermus caldophilus | - |
dc.subject.keyword | nagA gene | - |
dc.subject.local | N-acetylglucosamine 6-phosphate deacetylase | - |
dc.subject.local | thermus caldophillus | - |
dc.subject.local | Thermus caldophilus | - |
dc.subject.local | thermus caldophilus | - |
dc.subject.local | nagA gene | - |
dc.description.journalClass | Y | - |
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