Development of an enzymatic system for the production of dopamine from catechol, pyruvate, and ammonia

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dc.contributor.authorSeung Goo Lee-
dc.contributor.authorSeung Pyo Hong-
dc.contributor.authorMoon Hee Sung-
dc.date.accessioned2017-04-19T08:56:19Z-
dc.date.available2017-04-19T08:56:19Z-
dc.date.issued1999-
dc.identifier.issn0141-0229-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4802-
dc.description.abstractTo produce dopamine from catechol, pyruvate, and ammonia, an enzymatic process consisting of a two-step reaction, catechol → L-DOPA → dopamine, was developed. For the first reaction step to synthesize L-DOPA, tyrosine phenol-lyase of Symbiobacterium sp. SC-1 was used successfully as a biocatalyst, resulting in the high conversion yield of 92%. Two aromatic amino acid decarboxylases, rat liver L-DOPA decarboxylase and Streptoccus faecalis tyrosine decarboxylase (TDC), were tested for the subsequent step to produce dopamine. In investigating the effect of L-DOPA concentration, a serious substrate inhibition of L-DOPA decarboxylase activity was observed at concentrations over 1 mM, while no inhibition was detected for TDC up to 40 mM L-DOPA. Therefore, the TDC of S. faecalis was selected as the biocatalyst for the second reaction step. Enzymatic conversion of L-DOPA to dopamine was carried out in a reactor controlling the reaction pH with an HCl solution containing pyridoxal 5'-phosphate, to compensate for the loss of pyridoxal 5'-phosphate by an enzyme-catalyzed side reaction, i.e. decarboxylation-dependent transamination. When the enzyme reactor was operated at 37°C for 12 h, 100 mM of L-DOPA was converted to dopamine with the conversion yield of 100%. Simultaneous reactions of tyrosine phenol-lyase and TDC were tested for direct synthesis of dopamine, but the productivity was much lower than the separated two-step reactions.-
dc.publisherElsevier-
dc.titleDevelopment of an enzymatic system for the production of dopamine from catechol, pyruvate, and ammonia-
dc.title.alternativeDevelopment of an enzymatic system for the production of dopamine from catechol, pyruvate, and ammonia-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number0-
dc.citation.endPage302-
dc.citation.startPage298-
dc.citation.volume25-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorSeung Pyo Hong-
dc.contributor.affiliatedAuthorMoon Hee Sung-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName성문희-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 25, pp. 298-302-
dc.identifier.doi10.1016/S0141-0229(99)00071-X-
dc.subject.keywordDopamine-
dc.subject.keywordTyrosine phenol-lyase-
dc.subject.keywordTyrosine decarboxylase-
dc.subject.keywordSymbiobacterium-
dc.subject.keywordStreptococcus faecalis-
dc.subject.localDopamine-
dc.subject.localdopamine-
dc.subject.localTyrosine phenollyase-
dc.subject.localtyrosine phenol lyase-
dc.subject.localtyrosine phenol-lyase-
dc.subject.localTyrosine phenol-lyase-
dc.subject.localTyrosine decarboxylase-
dc.subject.localsymbiobacterium-
dc.subject.localSymbiobacterium-
dc.subject.localStreptococcus faecalis-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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