NMR solution conformation of and antitoxic analogue of α-conotoxin GI: identification of a common nicotinic acetylcholine receptor α₁-subunit binding surface for small ligans and α-conotoxins

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Title
NMR solution conformation of and antitoxic analogue of α-conotoxin GI: identification of a common nicotinic acetylcholine receptor α₁-subunit binding surface for small ligans and α-conotoxins
Author(s)
K Hun Mok; Kyou Hoon Han
Bibliographic Citation
Biochemistry, vol. 38, no. 37, pp. 11895-11904
Publication Year
1999
Abstract
The three-dimensional solution conformation of an 11-residue antitoxic analogue of α-conotoxin GI, des-Glu1-[Cys3Ala]-des-Cys13-conotoxin GI (CANPACGRHYS-NH2, designated 'GI-15' henceforth), has been determined using two-dimensional 1H NMR spectroscopy. The disulfide loop region (1C-6C) and the C-terminal tail (8R-11S) are connected by a flexible hinge formed near 7G, and the pairwise backbone rmsds for the former and the latter are 0.58 and 0.65 ?, respectively. Superpositioning GI-15 with the structure of α- conotoxin GI shows that the two share an essentially identical fold in the common first disulfide loop region (1C-6C). However, the absence of the second disulfide loop in GI-15 results in segmental motion of the C-terminal half, causing the key receptor subtype selectivity residue 8R (Arg9 in α- conotoxin GI) to lose its native spatial orientation. The combined features of structural equivalence in the disulfide loop and a mobile C-terminal tail appear to be responsible for the activity of GI-15 as a competitive antagonist against native toxin. Electrostatic surface potential comparisons of the first disulfide region of GI-15 with other α-conotoxins or receptor- bound states of acetylcholine and d-tubocurarine show a common protruding surface that may serve as the minimal binding determinant for the neuromuscular acetylcholine receptor α1-subunit. On the basis of the original 'Conus toxin macrosite model' [Olivera, B. M., Rivier, J., Scott, J. K., Hillyard, D. R., and Cruz, L. J. (1991) J. Biol. Chem. 266, 1923-1936], we propose a revised binding model which incorporates these results.
ISSN
0006-2960
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/bi990558n
Type
Article
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1. Journal Articles > Journal Articles
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