High-level expression of human lactoferrin in milk of transgenic mice using genomic lactoferrin sequence

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Title
High-level expression of human lactoferrin in milk of transgenic mice using genomic lactoferrin sequence
Author(s)
Sun Jung Kim; Bo Hwa Sohn; Sang Kyun Jeong; Kyung Won Pak; Jung Sun Park; In Young Park; Tae Hoon Lee; Young Hee Choi; Chul Sang Lee; Yong Mahn Han; Dae Yeul Yu; Kyung Kwang Lee
Bibliographic Citation
Journal of Biochemistry, vol. 126, pp. 320-325
Publication Year
1999
Abstract
In our previous study, transgenic mice were generated that expressed human lactoferrin (hLF) in milk using cDNA under control of the 2 kb bovine β-casein promoter. The expression level of the protein in milk of 7 mice ranged from 1 to 200 μg/ml; 1 to 34 μg/ml in 6 mice and 200 μg/ml in 1 mouse. With the aim of inducing higher expression of the protein, we constructed an expression cassette comprised of 10 kb of the bovine β-casein gene promoter and the hLF genomic sequence in place of the cDNA. The hLF genomic sequence of about 27 kb, spanning 23 kb of the entire coding region and 4 kb of the 3'-flanking sequence, was placed downstream the bovine β-casein promoter. In total, 8 transgenic mice were generated from 31 mice (transgenic rate of 25.8%) born from the embryos microinjected with the 40-kb hLF expression cassette. Mammary-specific expression of the transgene was addressed by performing Northern hybridization of the total RNAs from various tissues of transgenic mice. Immunoblot analysis showed that the recombinant protein expressed in milk has the same molecular weight as the native protein. The amount of the protein in milk of 5 mice ranged from 60 to 6600 μg/ml when judged by ELISA analysis. Three mice expressed the protein at the level higher than 500 μg/ml. These data suggest that the genomic lactoferrin sequence represents a valuable element for the efficient expression of the protein in milk of transgenic animals.
Keyword
bovine β-casein genehuman lactoferrin genemammary glandmilktransgenic mice
ISSN
0021-924X
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/oxfordjournals.jbchem.a022452
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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