Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp. SY16

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Title
Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp. SY16
Author(s)
Hee Sik Kim; Byung Dae Yoon; Dong Ho Choung; Hee Moak Oh; T Katsuragi; Y Tani
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 52, no. 5, pp. 713-721
Publication Year
1999
Abstract
One yeast strain, SY16, was selected as a potential producer of a biosurfactant, and identified as a Candida species. A biosurfactant produced from Candida sp. SY16 was purified and confirmed to be a glycolipid. This glycolipid-type biosurfactant lowered the surface tension of water to 29 dyne/cm at critical micelle concentration of 10 mg/l (1.5 x 10-5 M), and the minimum interfacial tension was 0.1 dyne/cm against kerosene. Thin-layer and high-pressure liquid chromatography studies demonstrated that the glycolipid contained mannosylerythritol as a hydrophilic moiety. The hydrophilic sugar moiety of the biosurfactant was determined to be β-D- mannopyranosyl-(1 → 4)-O-mesoerythritol by nuclear magnetic resonance (NMR) and fast atom bombardment mass-spectroscopy analyses. The hydrophobic moiety, fatty acids, of the biosurfactant was determined to be hexanoic, dodecanoic, tetradecanoic, and tetradecenoic acid by gas chromatography-mass spectroscopy. The structure of the native biosurfactant was determined to be 6-O-acetyl-2,3-di-O-alkanoyl-β-D-mannopyranosyl-(1 → 4)-O-meso-erythritol by NMR analyses. We newly determined that an acetyl group was linked to the C-6 position of the D-mannose unit in the hydrophilic sugar moiety.
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s002530051583
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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